rabbit polyclonal antibody against β1 adrenergic receptor Search Results


itgb1 antibody affinity biosciences  (Affinity Biosciences)
90
Affinity Biosciences itgb1 antibody affinity biosciences
Immunohistochemical staining and western blot following epithelial interstitial transformation (EMT) induced by silica in rats. A , C , and E , Representative images of E-cadherin, vimentin, and <t>ITGB1</t> positive expression in lung tissue (scale bars 50 μm). The arrows indicate E-cadherin-, vimentin-, and ITGB1-positive cells in IHC. B , D , and F , Statistical analysis of A , C , and E . G , Western blotting was used to detect the expression of EMT markers and ITGB1 in rat lung tissues of the control and experimental groups. H , Protein levels of E-cadherin, vimentin, and ITGB1. Data are reported as means±SD (n=3). *P<0.05 vs saline control group; ns: not significant (ANOVA).
Itgb1 Antibody Affinity Biosciences, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/itgb1 antibody affinity biosciences/product/Affinity Biosciences
Average 90 stars, based on 1 article reviews
itgb1 antibody affinity biosciences - by Bioz Stars, 2026-02
90/100 stars
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rabbit anti-guanylate cyclase β 1 polyclonal antibody  (Cayman Chemical)
90
Cayman Chemical rabbit anti-guanylate cyclase β 1 polyclonal antibody
Immunohistochemical staining and western blot following epithelial interstitial transformation (EMT) induced by silica in rats. A , C , and E , Representative images of E-cadherin, vimentin, and <t>ITGB1</t> positive expression in lung tissue (scale bars 50 μm). The arrows indicate E-cadherin-, vimentin-, and ITGB1-positive cells in IHC. B , D , and F , Statistical analysis of A , C , and E . G , Western blotting was used to detect the expression of EMT markers and ITGB1 in rat lung tissues of the control and experimental groups. H , Protein levels of E-cadherin, vimentin, and ITGB1. Data are reported as means±SD (n=3). *P<0.05 vs saline control group; ns: not significant (ANOVA).
Rabbit Anti Guanylate Cyclase β 1 Polyclonal Antibody, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-guanylate cyclase β 1 polyclonal antibody/product/Cayman Chemical
Average 90 stars, based on 1 article reviews
rabbit anti-guanylate cyclase β 1 polyclonal antibody - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
rabbit anti-human β1 and β2-adrenergic receptor polyclonal antibody  (Sangon Biotech)
90
Sangon Biotech rabbit anti-human β1 and β2-adrenergic receptor polyclonal antibody
Immunohistochemical staining and western blot following epithelial interstitial transformation (EMT) induced by silica in rats. A , C , and E , Representative images of E-cadherin, vimentin, and <t>ITGB1</t> positive expression in lung tissue (scale bars 50 μm). The arrows indicate E-cadherin-, vimentin-, and ITGB1-positive cells in IHC. B , D , and F , Statistical analysis of A , C , and E . G , Western blotting was used to detect the expression of EMT markers and ITGB1 in rat lung tissues of the control and experimental groups. H , Protein levels of E-cadherin, vimentin, and ITGB1. Data are reported as means±SD (n=3). *P<0.05 vs saline control group; ns: not significant (ANOVA).
Rabbit Anti Human β1 And β2 Adrenergic Receptor Polyclonal Antibody, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-human β1 and β2-adrenergic receptor polyclonal antibody/product/Sangon Biotech
Average 90 stars, based on 1 article reviews
rabbit anti-human β1 and β2-adrenergic receptor polyclonal antibody - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Immunohistochemical staining and western blot following epithelial interstitial transformation (EMT) induced by silica in rats. A , C , and E , Representative images of E-cadherin, vimentin, and ITGB1 positive expression in lung tissue (scale bars 50 μm). The arrows indicate E-cadherin-, vimentin-, and ITGB1-positive cells in IHC. B , D , and F , Statistical analysis of A , C , and E . G , Western blotting was used to detect the expression of EMT markers and ITGB1 in rat lung tissues of the control and experimental groups. H , Protein levels of E-cadherin, vimentin, and ITGB1. Data are reported as means±SD (n=3). *P<0.05 vs saline control group; ns: not significant (ANOVA).

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Inhibition of the ITGB1 gene attenuates crystalline silica-induced pulmonary fibrosis via epithelial-mesenchymal transformation

doi: 10.1590/1414-431X2024e13486

Figure Lengend Snippet: Immunohistochemical staining and western blot following epithelial interstitial transformation (EMT) induced by silica in rats. A , C , and E , Representative images of E-cadherin, vimentin, and ITGB1 positive expression in lung tissue (scale bars 50 μm). The arrows indicate E-cadherin-, vimentin-, and ITGB1-positive cells in IHC. B , D , and F , Statistical analysis of A , C , and E . G , Western blotting was used to detect the expression of EMT markers and ITGB1 in rat lung tissues of the control and experimental groups. H , Protein levels of E-cadherin, vimentin, and ITGB1. Data are reported as means±SD (n=3). *P<0.05 vs saline control group; ns: not significant (ANOVA).

Article Snippet: The following products were also used to carry out the experiments: restriction endonuclease (New, England Biolabs, USA); Protein ladder (Thermo Fisher Scientific, 26617, USA); DNA marker (Beijing Quanshi Gold Biotechnology Co., Ltd., BM121, China); plasmid extraction kit (American Omega, D6943-01*, USA); agarose gel recovery kit (Omega, D250-01); ITGB1 antibody (Affinity Biosciences, USA); E-cadherin antibody (Affinity Biosciences); vimentin antibody (Affinity Biosciences); ILK antibody (Affinity Biosciences); Snail antibody (Affinity Biosciences); GAPDH antibody (Beijing Boosen Biotechnology Company, China); fetal bovine serum (Gibco, USA); and DMEM high glucose medium (Gibco).

Techniques: Immunohistochemical staining, Staining, Western Blot, Transformation Assay, Expressing, Control, Saline

Validation of ITGB1 knockdown (KD) in BEAS-2B cells. A , sgRNA targets are located before and after the second exon of the ITGB1 gene. The figure shows the sequences containing the gRNA primers T1 and T2. B , After 24 h, the control (WT) and experimental groups were examined under a microscope. BEAS-2B cells appeared cubic and polygon-shaped, typical of respiratory epithelial cells. When ITGB1 was knocked down, BEAS-2B cells were short and fusiform. Scale bars 100 μm. C , The vitality of cells was determined using a counting cell test in the presence and absence of the ITGB1 gene (n=3). D , Cell proliferation assay was performed using the Cell Counting Kit-8 according to the manufacturer's instruction. E , Agarose gel electrophoresis confirmed the KD of ITGB1. F , Western blot assay was used to detect ITGB1 protein expression in the control and experimental groups. Data are reported as means±SD. *P<0.05 (ANOVA).

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Inhibition of the ITGB1 gene attenuates crystalline silica-induced pulmonary fibrosis via epithelial-mesenchymal transformation

doi: 10.1590/1414-431X2024e13486

Figure Lengend Snippet: Validation of ITGB1 knockdown (KD) in BEAS-2B cells. A , sgRNA targets are located before and after the second exon of the ITGB1 gene. The figure shows the sequences containing the gRNA primers T1 and T2. B , After 24 h, the control (WT) and experimental groups were examined under a microscope. BEAS-2B cells appeared cubic and polygon-shaped, typical of respiratory epithelial cells. When ITGB1 was knocked down, BEAS-2B cells were short and fusiform. Scale bars 100 μm. C , The vitality of cells was determined using a counting cell test in the presence and absence of the ITGB1 gene (n=3). D , Cell proliferation assay was performed using the Cell Counting Kit-8 according to the manufacturer's instruction. E , Agarose gel electrophoresis confirmed the KD of ITGB1. F , Western blot assay was used to detect ITGB1 protein expression in the control and experimental groups. Data are reported as means±SD. *P<0.05 (ANOVA).

Article Snippet: The following products were also used to carry out the experiments: restriction endonuclease (New, England Biolabs, USA); Protein ladder (Thermo Fisher Scientific, 26617, USA); DNA marker (Beijing Quanshi Gold Biotechnology Co., Ltd., BM121, China); plasmid extraction kit (American Omega, D6943-01*, USA); agarose gel recovery kit (Omega, D250-01); ITGB1 antibody (Affinity Biosciences, USA); E-cadherin antibody (Affinity Biosciences); vimentin antibody (Affinity Biosciences); ILK antibody (Affinity Biosciences); Snail antibody (Affinity Biosciences); GAPDH antibody (Beijing Boosen Biotechnology Company, China); fetal bovine serum (Gibco, USA); and DMEM high glucose medium (Gibco).

Techniques: Biomarker Discovery, Knockdown, Control, Microscopy, Proliferation Assay, Cell Counting, Agarose Gel Electrophoresis, Western Blot, Expressing

Effect of knockdown (KD) ITGB1 on integrin/ILK signaling pathway and epithelial interstitial transformation (EMT) in silica-stimulated BEAS-2B cells. A , Schematic diagram of the experimental cells. B , Western blotting detected the expression of EMT markers in the ITGB1-knocked down BEAS-2B cells. C , The expression of integrin/ILK signaling pathway markers in BEAS-2B cells treated with ITGB1 -/- was measured by western blotting. D , The protein levels of E-cadherin, vimentin, ITGB1, ILK, and Snail were quantified by the ImageJ 6.0 software. Data are reported as means±SD (n=3). *P<0.05 vs control group, # P<0.05 vs SiO 2 group (ANOVA). E , Confocal microscopy was used to observe the immunofluorescence of E-cadherin and vimentin. Scale bars 50 μm.

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Inhibition of the ITGB1 gene attenuates crystalline silica-induced pulmonary fibrosis via epithelial-mesenchymal transformation

doi: 10.1590/1414-431X2024e13486

Figure Lengend Snippet: Effect of knockdown (KD) ITGB1 on integrin/ILK signaling pathway and epithelial interstitial transformation (EMT) in silica-stimulated BEAS-2B cells. A , Schematic diagram of the experimental cells. B , Western blotting detected the expression of EMT markers in the ITGB1-knocked down BEAS-2B cells. C , The expression of integrin/ILK signaling pathway markers in BEAS-2B cells treated with ITGB1 -/- was measured by western blotting. D , The protein levels of E-cadherin, vimentin, ITGB1, ILK, and Snail were quantified by the ImageJ 6.0 software. Data are reported as means±SD (n=3). *P<0.05 vs control group, # P<0.05 vs SiO 2 group (ANOVA). E , Confocal microscopy was used to observe the immunofluorescence of E-cadherin and vimentin. Scale bars 50 μm.

Article Snippet: The following products were also used to carry out the experiments: restriction endonuclease (New, England Biolabs, USA); Protein ladder (Thermo Fisher Scientific, 26617, USA); DNA marker (Beijing Quanshi Gold Biotechnology Co., Ltd., BM121, China); plasmid extraction kit (American Omega, D6943-01*, USA); agarose gel recovery kit (Omega, D250-01); ITGB1 antibody (Affinity Biosciences, USA); E-cadherin antibody (Affinity Biosciences); vimentin antibody (Affinity Biosciences); ILK antibody (Affinity Biosciences); Snail antibody (Affinity Biosciences); GAPDH antibody (Beijing Boosen Biotechnology Company, China); fetal bovine serum (Gibco, USA); and DMEM high glucose medium (Gibco).

Techniques: Knockdown, Transformation Assay, Western Blot, Expressing, Software, Control, Confocal Microscopy, Immunofluorescence

The effect of knockdown (KD) integrin ITGB1 on the migration of BEAS-2B cells. A , BEAS-2B cells were migrated at 0 h and 24 h in each group (scale bars 100 μm). B , The migration of BEAS-2B cells in each group was quantified by the ImageJ 6.0 software. Data are reported as means±SD (n=3). *P<0.05 vs control group, # P<0.05 vs SiO 2 group (ANOVA).

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Inhibition of the ITGB1 gene attenuates crystalline silica-induced pulmonary fibrosis via epithelial-mesenchymal transformation

doi: 10.1590/1414-431X2024e13486

Figure Lengend Snippet: The effect of knockdown (KD) integrin ITGB1 on the migration of BEAS-2B cells. A , BEAS-2B cells were migrated at 0 h and 24 h in each group (scale bars 100 μm). B , The migration of BEAS-2B cells in each group was quantified by the ImageJ 6.0 software. Data are reported as means±SD (n=3). *P<0.05 vs control group, # P<0.05 vs SiO 2 group (ANOVA).

Article Snippet: The following products were also used to carry out the experiments: restriction endonuclease (New, England Biolabs, USA); Protein ladder (Thermo Fisher Scientific, 26617, USA); DNA marker (Beijing Quanshi Gold Biotechnology Co., Ltd., BM121, China); plasmid extraction kit (American Omega, D6943-01*, USA); agarose gel recovery kit (Omega, D250-01); ITGB1 antibody (Affinity Biosciences, USA); E-cadherin antibody (Affinity Biosciences); vimentin antibody (Affinity Biosciences); ILK antibody (Affinity Biosciences); Snail antibody (Affinity Biosciences); GAPDH antibody (Beijing Boosen Biotechnology Company, China); fetal bovine serum (Gibco, USA); and DMEM high glucose medium (Gibco).

Techniques: Knockdown, Migration, Software, Control

Role of the integrin/ILK signaling pathway in animal and cell models of epithelial interstitial transformation (EMT). In vivo , silica induced EMT in rats. In vitro , silica stimulated BEAS-2B cells to promote EMT by upregulating the integrin/ILK signaling pathway. ITGB1 knockdown reduced EMT by downregulating the integrin/ILK pathway.

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Inhibition of the ITGB1 gene attenuates crystalline silica-induced pulmonary fibrosis via epithelial-mesenchymal transformation

doi: 10.1590/1414-431X2024e13486

Figure Lengend Snippet: Role of the integrin/ILK signaling pathway in animal and cell models of epithelial interstitial transformation (EMT). In vivo , silica induced EMT in rats. In vitro , silica stimulated BEAS-2B cells to promote EMT by upregulating the integrin/ILK signaling pathway. ITGB1 knockdown reduced EMT by downregulating the integrin/ILK pathway.

Article Snippet: The following products were also used to carry out the experiments: restriction endonuclease (New, England Biolabs, USA); Protein ladder (Thermo Fisher Scientific, 26617, USA); DNA marker (Beijing Quanshi Gold Biotechnology Co., Ltd., BM121, China); plasmid extraction kit (American Omega, D6943-01*, USA); agarose gel recovery kit (Omega, D250-01); ITGB1 antibody (Affinity Biosciences, USA); E-cadherin antibody (Affinity Biosciences); vimentin antibody (Affinity Biosciences); ILK antibody (Affinity Biosciences); Snail antibody (Affinity Biosciences); GAPDH antibody (Beijing Boosen Biotechnology Company, China); fetal bovine serum (Gibco, USA); and DMEM high glucose medium (Gibco).

Techniques: Transformation Assay, In Vivo, In Vitro, Knockdown